Verone Chambers and Jura Levy saw their Olympic hopes end for 2012 at the Jamaican Olympic Trials.
Chambers finished fifth in her heat of the 400 meters and 11th overall to miss the finals in 52.90 seconds.
Levy, who has had knee problems, did not run in the 200 meters.
The last event involving former OBU athletes begins Sunday. Nickeisha Wilson, who has already made the Olympic team in the 400 hurdles, will be in the field for the 100 meter hurdles, along with former OBU teammate Latoya Greaves.